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| GenEscort系列转染试剂在Google学术搜索上的应用文章统计情况 |
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（2018年9月不完全统计）
H Chen, H Sun, F You, W Sun, X Zhou, L Chen, J Yang… - Cell, 2011 - Elsevier STAT6 plays a prominent role in adaptive immunity by transducing signals from extracellular cytokines. We now show that STAT6 is required for innate immune sign.
J Zhang, F Ji, Y Liu, X Lei, H Li, G Ji… - Journal of …, 2014 - Soc Neuroscience
Interleukin-33 increases antibacterial defense by activation of inducible nitric oxide synthase in skin
C Li, H Li, Z Jiang, T Zhang, Y Wang, Z Li, Y Wu… - PLoS …, 2014 - journals.plos.org Author Summary Interleukin-33 (IL-33) is associated with multiple diseases, including asthma, rheumatoid arthritis, tissue injuries and infections. Although IL-33 has been indicated to be involved in wound infections, little is known about how IL-33 is regulated as a mechanism to …
J Cheng, W Li, B Kang, Y Zhou, J Song, S Dan… - Nature …, 2015 - nature.com Article.
X Zhou, F You, H Chen, Z Jiang - Cell research, 2012 - nature.com … antibiotics. Transient transfection was carried out by the standard calcium phosphate precipitation method. Poly(I:C) (1 μg/ml) was transfected using GenEscort II (Wisegen) according to the manufacturer's instructions. Where …
W Qiu, N Che, X Feng, M Xia, H Wang… - European journal of …, 2009 - Wiley Online Library Skip to Main Content …
X Wang, J Tang, L Xing, G Shi, H Ruan… - The EMBO …, 2010 - emboj.embopress.org
Skip to main content. Advertisement. Other Publications: EMBO Press; The EMBO Journal (Home); EMBO reports; EMBO Molecular Medicine; Molecular Systems Biology. Institution: Google Indexer. Login. Search. Search for this keyword. Advanced Search. Journal …
X Lv, H Jiang, B Li, Q Liang, S Wang, Q Zhao, J Jiao - Scientific reports, 2014 - nature.com … incubator. The cells were plated and cultured for 24 hours before plasmid transfection. Plasmid transfection was performed using GenEscort I (Nanjing Wisegen
Biotechnology) according to the manufacturer's instructions. The …
H Zhuang, W Jiang, X Zhang, F Qiu, Z Gan… - Journal of molecular …, 2013 - Springer
… When tumors reached a size of approximately 5 × 5 mm 2 , the mice were arbitrarily assigned to different groups to receive intratumoral injections of 20 μl GenEscort™ Reagent (Wisegen Biotechnology, Nanjing, China) complexed with psiLUC, psiHSP27, psiHSP70, or PBS …
JX Yang, L Hua, YQ Li, YY Jiang, D Han… - Journal of …, 2015 - Soc Neuroscience
… US. Recombinant Human sIL-6 Rα was purchased from PEPROTECH (New Jersey, USA). GenEscort TM Transfection Reagent was purchased from Wisegen Biotechnology Corporation (Nanjing, China). Lipofectamine transfection …
K Lü, D Zeng, Y Zhang, L Xia, L Xu, DL Kaplan… - Annals of biomedical …, 2011 - Springer
The study was to explore the effects of BMP-2 gene modified canine bone marrow stromal cells (bMSCs) mediated by a nonviral PEI derivative (GenEscort™ II) in promoting bone formation in vitro and in v …
K Xu, Y Zhou, W Qiu, X Liu, M Xia, L Liu, X Liu, D Zhao… - Immunobiology, 2011 - Elsevier … The cultured GMCs were seeded in 6-well plates at a density of 3 × 10 5 per well over night, and were transfected with 4 μg of a luciferase reporter (pGL3 reconstructed with different regions
of Gadd45α or KLF6 promoter) and 4 μg PRL-SV40, using GenEscort™ III according to …
Phosphorylation of the Twist1-family basic helix-loop-helix transcription factors is involved in pathological cardiac remodeling
S Lu, J Nie, Q Luan, Q Feng, Q Xiao, Z Chang, C Shan… - PloS one, 2011 - journals.plos.org Background The Twist1-family basic helix-loop-helix (bHLH) transcription factors including Twist1, Hand1 and Hand2, play an essential role in heart development and are implicated in pathological heart remodeling. Previously, it was reported that these bHLH transcription factors …
J Nie, M Jiang, X Zhang, H Tang, H Jin, X Huang… - Cell reports, 2015 - Elsevier
RNA G-quadruplexes (G4s) play important roles in RNA biology. However, the function and regulation of mRNA G-quadruplexes in embryonic development remain elusiv.
J Li, P Hu, K Li, Q Zhao - The Anatomical Record: Advances in …, 2012 - Wiley Online Library
… Electrophoretic Mobility Shift Assays. To perform EMSA, we first transfected 293T cells with 4 μg of pWI-Ef1a-RARαb (previously named RARα2.B; Perz-Edwards et al.,2001) or vehicle vector
pWI-Ef1a in 100-mm dish using GenEscort II (Wisegen, Nanjing, China) …
and behaviors, and disruption of circadian rhythmicity can profoundly.
PMS1077 sensitizes TNF-α induced apoptosis in human prostate cancer cells by blocking NF-κB signaling pathway
J Shi, J Chen, N Serradji, X Xu, H Zhou, Y Ma, Z Sun… - PLoS …, 2013 - journals.plos.org
… TNF-α was purchased from Pepro Tech. GenEscort™ Transfection Reagent was purchased from Wisegen Biotechnology Corporation (Nanjing, China). Transfection and luciferase reporter assay. DU145 and PC3 cells were seeded into 60 mm tissue culture dishes, respectively …
X Jiang, J Zhang, M Xia, W Qiu, H Wang… - Cellular & molecular …, 2010 - nature.com …GenEscort III was supplied by Wisegen (Nanjing, China) … Transient transfection of ATF3 expression plasmids or ATF3 shRNA expression plasmids into the cultured GMCs was conducted using GenEscort III according to the manufacturer's instructions …
X Liu, Y Lan, D Zhang, K Wang, Y Wang… - American journal of …, 2014 - ncbi.nlm.nih.gov … The mice received direct local injections of the GenEscort™ Reagent (Wisegen Biotechnology, Nanjing, China) and plasmid complexes (5 ug plasmid per mouse). Injections were repeated every 3 days for a total of three times …
HM Chen, K Yu, X Tang, Z Bao, T Jiang, XL Fan… - FEBS letters, 2015 - Elsevier
… Cells were seeded in 6-well plates and allowed to grow overnight to 60–80% confluence. ZM fusion vectors (2 μg) were individually mixed with 4-μl GenEscort II (Wisegen, Nanjing, China), incubated for 15 min at room temperature, and added to the cell medium …
Z Xiong, X Dong, P Sun, Y Zhang - Sheng wu yi xue gong cheng …, 2013 - europepmc.org
… vitro. Tumor xenografts were transfected with siE7 and GenEscort III by nanopatch … vivo. We transfected GenEscort III and siE7 of Different concentration into tumor xenografts respectively by nanopatch and intraperitoneal injection …
S Deng, Y Zhou, D Ouyang, J Xiong… - Biomedical …, 2014 - spandidos-publications.com
… Louis, MO, USA) without serum and mixed gently. Preparation of the GenEscort III complex. A
total of 36 µl GenEscort III (Wisegen Biotechnology Corp., Nanjing, China) were diluted in 1.5 ml DMEM without serum, mixed gently and incubated for 5 min at room temperature …
X Chang, G Cao, K Wang, X Zhang… - Acta biochimica et …, 2015 - academic.oup.com
… Logan, USA). Cells (1 × 10 5 per well) were seeded in 6-well plate, and cultured
for 24 h. The mixture of 0.2 μg DNA and 1 μl liposome (GenEscort III Wisegen, Nanjing, China) was added into each well. Confocal images were …
Y Zhai, G Shu, X Zhu, Z Zhang, X Lin… - Journal of animal …, 2012 - jasbsci.biomedcentral.com … Transient transfections were performed using GenEscort III (Wisegen, China) with approximately 1.5x10 5 cells/well in 24-well dishes and 2 μg of each DNA. Plasmids were purified with the EZNA TM EndoFee Plasmid Kit (Omega, China) …
K Shan, R Pang, C Zhao, X Liu, W Gao… - Cellular & molecular …, 2017 - nature.com
… A Neon transfection system was provided by Invitrogen. GenEscort III was from Wisegen (Nanjing, China) … HEK293T cells were transfected with corresponding miRNAs or plasmids using GenEscort III according to the manufacturer's instructions …
熊倬， 董晓静， 孙培文， 张莹 - 生物医学工程学杂志, 2013 - cqvip.com
… 宫颈癌动物模型），分析siRNA对HPV基因表达的抑制作用，进而探讨纳米板在动物水平上转导siRNA 的优越性及转导的最佳作用时间和浓度。合成针对HPVl8E7mRNA的siRNA（简称siE7），细胞实验检测 siE7转录后的沉默效果。采用纳米板转导siE7与GenEscort（转染试剂）复合物进入 …
T Hu, Z Wang, F Zeng, X Chen, Z Gu, L Zheng… - Journal of Huazhong …, 2010 - Springer … The intratesticular injection procedure was previ- ously described[8, 9]. In PEI group, the testes were in- jected with PEI. In vector and TSEG-2 groups, the plas- mid DNA (pEGFP-N1 or
pEGFP-TSEG-2) was mixed with PEI (GenEscort™ Ⅲ, Nanjing Wisegen Biotech- nology Co … 被引用次数：2 相关文章 所有 8 个版本
FAN Li, LUO Su, J Rui - Journal of Beihua University (Natural …, 2008 - en.cnki.com.cn
… and hTERT was detected by RT-PCR;cell proliferation by MTT;telomerase activity by TRAP;cell apoptosis by FCM.Results Effects of telomerase hTERT ribozyme on MCF-7 cell is tested.pcDNA3.1(+)-RZ had been successful transfected into MCF-7 cell by GenEscort TMII.We …
K Lü, D Zeng, W Zhang, L Xia, L Xu… - Cell biology …, 2012 - Wiley Online Library
… new bone formation effects of CPC (calcium phosphate cement) seeded with pBMP-2 (plasmids containing bone morphogenetic protein-2 gene) transfected canine bMSCs (bone marrow stromal cells) mediated by a non-viral PEI (polyethylenimine) derivative (GenEscort™ II) in …
车楠， 邱文， 夏梅， 赵聃， 王迎伟 - 南京医科大学学报: 自然科学版, 2009 - cqvip.com
… GMC）中过表达及沉默Gadd45γ基因的情况。方法：用DNA重组技术将针对大鼠Gadd45γ基因的cds 区序列或针对其不同位点所设计的4对shRNA序列分别克隆到真核表达质粒pcDNA3．1／HA或pGCsi． U6．neo．GFP中。在酶切分析及序列测定正确后，用GenEscort^TM Ⅲ …
张娜， 刘璐， 窦玥莹， 王真， 宋丹青， 李电东… - 中国医药生物 …, 2015 - cmbp.net.cn
… T4 DNA 连接酶、限制性内切酶、 PCR 产物纯化试剂盒、胶回收试剂盒等购自大连宝 生物公司；DMEM 高糖培养基和胎牛血清购自美 国Hyclone 公司；caspase-3 底物DEVD-AFC、 GSK-3β 抗体、β-actin 抗体和羊抗兔或小鼠二抗购 自美国Santa Cruz 公司；GenEscort II 转染试剂购 …
黄伟翼， 靖大道， 沈赞 - 肿瘤, 2008 - tumorsci.org
… 1 材料与方法 1.1 实验材料 人乳腺癌细胞株MCF-7由复旦大学附属肿瘤医院邵志敏教授惠赠,用含
10%胎牛血清和2 mmol/L谷氨酸的DMEM培养液,于体积分数为5%的CO 2 、37℃条件下培养。Actin、
PI3K和FoxO3a多克隆抗体购自美国Upstate Biothechnology公司。GenEscort TM Ⅲ …
郭红艳， 孙晓杰， 刘秀财， 刘波， 李淑艳， 张冬梅 - 中华肿瘤防治杂志, 2016 - cqvip.com
… 参与细胞增殖、分化和物质转运等生命活动,在某些肿瘤形成中担任重要角色。本研究设计观察SGK3 过表达对乳腺癌细胞株MDA-MB-231细胞增殖和存活的影响,并初步探讨其作用的分子机制。 方法免疫组化方法检测乳腺癌组织中SGK3蛋白的表达;通过GenEscort TMⅠ介导用重组质粒 …
车楠 - 2009 - cdmd.cnki.com.cn
… mesangial cells, GMCs)中过表达及沉默Gadd45γ基因的情况。方法:用DNA重组技术将针对大鼠 Gadd45γ基因的cds区序列或针对其不同位点所设计的4对shRNA序列分别克隆到真核表达质粒 pcDNA3.1/HA或pGCsi.U6.neo.GFP中。在酶切分析及序列测定正确后,用GenEscort~(TM)Ⅲ …
钟发刚 - 成都: 四川农业大学, 2008 - cdmd.cnki.com.cn
… E2蛋白的真核重组质粒pVAXl-E2；经过PCR、酶切及测序表明：pProEX HTa-E2和pVAXl-E2 重组表达载体构建成功。将pProEX HTa-E2质粒转化大肠杆菌DH5、JM109和BL21并诱导表达， 表达目的蛋白用SDS-PAGE检测，目的蛋白没有表达；用GenEscort~(TM)试剂转染pVAil-E2质粒 …
X Sun, X Liu, B Liu, S Li, D Zhang… - Oncology …, 2016 - spandidos-publications.com … Each well was transfected with 3 µg pEGFP-N1-SGK3 or pEGFP-N1 using GenEscort™ I (Isegen, Nanjing, China) according to the manufacturer's instructions. Forty-eight hours after transfection,
the treated cells were harvested and SGK fusion protein analyses were performed …
GI Im - Journal of Biomedical Materials Research Part A, 2013 - Wiley Online Library
… osteogenic differentiation in vivo. 75. BMP‐2, PEI derivative (GenEscort™ II), Mouse
ectopic ossification, Enhanced osteogenic differentiation of canine MSCs and promotion of the ectopic new bone formation. 76. BMP‐2, cDNA …
X Bai, XP Wu, XL Wang, XR Wang, JL Yu… - Chinese Journal of …, 2010 - cabdirect.org
The T668 gene was amplified by RT-PCR from Trichinella spiralis NBL originated from pigs in Henan Province ISS534 and cloned into the eukaryotic expression plasmid pEGFP-N1. Then, the pEGFP-N1-T668 plasmid was transfected into BHK cells mediated by GenEscortTM II …
FL Zhang, W Dong, XY Wu, SQ Hu… - Molecular …, 2009 - spandidos-publications.com
… Binary complex of GenEscort Ⅲ (biodegradable hyperbranched Pei, Wisegen Biotechnology
corp., nanjing, Pr china) and SSos were prepared (17) at a ratio of 6:1 Pei nitrogen/SSo phosphate
(n/P=6). SSos and a corresponding amount of PEI solution were respectively diluted …
J Zhang, L Fang, X Zhu, Y Qiao, M Yu… - Cell biology …, 2012 - Wiley Online Library … Plasmids of FLAG-Ro52 and FLAG-Ro52ΔRING were provided by Dr Shigetsugu Hatakeyama (Hokkaido University, Japan). Transfection with GenEscort™ reagent (Wisegen Biotechnology Co.) is previously described (Dong et al., 2006). 2.3 RNAi (RNA interference) of Ro52 …
S Dan, B Kang, X Duan, YJ Wang - Biochemical and biophysical research …, 2015 - Elsevier
… For transfection, 293T cells were grown on 10 cm plates to a 30–50% confluence, and transfected with 5–10 μg of DNA per plate using GenEscort II reagent (Wisegen, Nanjing, China) with a ratio of 1:3 (w/v). 2.3. Plasmids. The …
X Wang, W Zhu, X Zhao… - … journal of molecular …, 2016 - spandidos-publications.com
… Subsequently, 3 µl GenEscort™ transfection reagent (Wisegen Biotechnology Co., Nanjing, China)
was diluted in 50 µl serum-free culture medium and then lightly shaken. Transfection
reagent-containing- and pEZX-miR-133a plasmid-containing solutions were then combined …
QJ Zeng, LN Wang, G Shu, SB Wang, XT Zhu… - Poultry …, 2014 - researchgate.net
… were plated in four 6-well plates and transfected respectively with 2 μg of the pcDNA3.1(-) -Decorin plasmid construct, 2 μg of the pcDNA3.1(-) empty vectors, 4 μg of pGPu6/GFP/Neo-sh-Decorin vectors, and 4 μg of pGPu6/GFP/Neo-sh-NC vectors by GenEscort TMII (Wisegen …
QJ Zeng, LN Wang, G Shu, SB Wang, XT Zhu… - Poultry …, 2013 - academic.oup.com Abstract. Decorin, a small leucine-rich proteoglycan as a component of the extracellular matrix, plays an important role in the skeletal muscle development. It.
X Cheng, QY Xi, S Wei, D Wu, RS Ye… - Journal of animal …, 2016 - academic.oup.com Abstract. Alteration of gene expression tightly regulates lipogenesis. Stearoyl-CoA desaturase-1 (SCD-1), a key enzyme in lipogenesis, catalyzes the conversion.
X He, J Wang, F Zhao, D Chen, J Chen… - Scandinavian …, 2013 - Wiley Online Library
… group. Each mouse was immunized subcutaneous (sc)/intramuscular (im) with 50 μl PBS or 50 μl DNA vaccine compound containing 10 μg pIRES-ESAT-6-gpi, the
20 μl GenEscort™ III transfection reagent (Wisegen Biotech. Co …
L Su, W Xia, T Shen, Q Liang, W Wang… - Nucleic acids …, 2018 - academic.oup.com Abstract. Astrocytes play crucial roles in the central nervous system, and defects in astrocyte function are closely related to many neurological disorders. St.
M Cao, L Bai, D Wang, Q Zhai, Y Li, J Hai… - … JOURNAL OF CLINICAL …, 2018 - ijcem.com
… To investigate the role of miRNA-33 on DN pathogenesis, the transfections of both miRNA-33 inhibitors (GenePharma Co., Ltd, Shanghai, China) (inhibitor group) and interfer- ence-free RNAs (control group) were perform- ed using a GenEscort TMIII (WiseGen Company …
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聚乙烯亚胺介导骨形态发生蛋白2 基因转染骨髓间充质干细胞的研究
方志辉， 谭金海， 简超 - 中华实验外科杂志, 2012
邓跃飞， 雷炳喜， 赵义营， 牛江涛 - 第三军医大学学报, 2011 - aammt.tmmu.edu.cn
… 南京慧基生物技术有限公司；兔抗人InG4抗体购自美国PTG 公司，羊抗兔IgG和即用型SABC 免疫组化试剂盒购自武汉博 士德生物工程有限公司；DMSO购自Sigma公司，MTT购自上海 捷瑞生物工程有限公司。 1.2 方法 1.2.1 细胞转染及转染后鉴定按Genescort说明书PEI按 1∶1比例向 …
QE Qi, QY Xi, RS Ye, T Chen, X Cheng, CY Li… - Growth hormone & IGF …, 2015 - Elsevier … transfection. On the following day, 100 ng of reporter plasmid along with 10 ng of control Renilla-luciferase plasmid (pRL-TK-Promega) was co-transfected using PEI (GenEscort™III, Nanjing Wisegen Biotechnology Co. Ltd., China) …
W Pang, Y Zhang, S Wang, A Jia, W Dong, C Cai… - Journal of lipid …, 2011 - ASBMB … Negative control shRNAs was used to assess non-specific gene silencing effects. The mammalian expression vector pRNA-U6.1 was used to express shRNAs in HSC-T6. ShRNAs were introduced
into HSC-T6 cells by GenEscort™ , according to the manufacturer's protocol. Ⅱ …
H Zhao, H Yu, J Zheng, N Ning, F Tang, Y Yang… - Gynecologic …, 2018 - Elsevier
… type (GAS5-Mut). The recombinant reporter gene plasmid GAS5-Wt or GAS5-Mut (500 ng) and miR-196a-5p mimic were co-transfected into ovarian cancer cells through GenEscort™ I transfection reagent. Synchronically, the …
Z Xia, K Jiang, T Liu, H Zheng, X Liu, X Zheng - Journal of pediatric surgery, 2013 - Elsev, ier Skip to main content …
L Li, Y Zhu, J Sun, T Chen, J Luo, G Shu, S Wang… - 2018 - preprints.org
… and pmirGLO-PAP-Del were co-transfected with the miR-125b-5p mimic or negative control (NC). GenEscort II (Huiji, Nanjing, China) were used to mediate the
transfection procedure according to the manufacturer's protocol …
W Xia, L Su, J Jiao - J Cell Biol, 2018 - jcb.rupress.org
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